Superresolution Spectroscopic Microscopy

In this talk, I will introduce spectroscopic photon localization microscopy, which is capable of simultaneously capturing the spatial distributions of photons emitted by individual molecules and their inherent spectroscopic signatures with sub-nm spectral resolution. As a result, the origins of photon emissions from different molecules can be identified according to their spectral differences. Using molecular identification, we can significantly extend the fundamental resolution limit of single molecule localization microscopy. Taking advantage of this unique capability, we demonstrated improvement in spatial resolution of PALM/STORM up to ten fold with selected fluorophores.